HES5 Antibody

• Catalog Number: orb1239428
• Category: Antibodies
• Description: HES5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5.The immunogen is located within the first 50 amino acids of HES5.
• Concentration: 1 mg/mL
• Dilution range: HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 18 kDa Observed: 17 kDa
• Target: HES5
• UniProt ID: Q5TA89
• NCBI: NP_001010926
• Storage: HES5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: HES5 antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Hairy and enhancer of split 5, hes family bHLH tra
• Note: For research use only
• Application notes: HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 µg/ml (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of HES5 in human spleen tissue with HES5 antibody at 5 µg/ml.

Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 µg/ml.

Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibody at 5 µg/mL.

Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibody at 20 µg/mL. Green: HES5 Antibody (orb1239428) Blue: DAPI staining.