Hepatocyte Specific Antigen Antibody

• Catalog Number: orb750007
• Category: Antibodies
• Description: mAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: The human hepatocellular carcinoma cell line SK-H1A9-2 was used as the immunogen for this Hepatocyte Specific Antigen antibody.
• UniProt ID: Not Known
• Tested applications: ELISA
• Dilution range: ELISA (For coating, purchase antibody without BSA)
• Application notes: The optimal dilution of the Prolactin Receptor antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: HSA133
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Hepatocyte Specific Antigen antibody is available for research use only.
• Note: For research use only

SDS-PAGE Analysis of Purified, BSA-Free Hepatocyte Specific Antigen Antibody (clone HSA133). Confirmation of Integrity and Purity of the Antibody.