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Heparanase 1 Antibody
Catalog Number: orb1258985
| Catalog Number | orb1258985 |
|---|---|
| Category | Antibodies |
| Description | Heparanase 1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Recombinant protein of human Heparanase 1 |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 50kDa/65kDa |
| Target | HPSE |
| UniProt ID | Q9Y251 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | HPSE, Endo-glucoronidase, Heparanase-1, Heparanase Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cells, using Heparanase 1 antibody (orb1258985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using Heparanase 1 antibody (orb1258985) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Heparanase 1 antibody (orb1258985) at dilution of 1:100. Blue: DAPI for nuclear staining.
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