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Item 1 of 4
Item 1 of 4
HELLS Antibody, KO Validated
Catalog Number: orb1274441
| Catalog Number | orb1274441 |
|---|---|
| Category | Antibodies |
| Description | HELLS Antibody, KO Validated |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, IP, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2). |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 120kDa |
| Target | HELLS |
| UniProt ID | Q9NRZ9 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | ICF4, LSH, Nbla10143, PASG, SMARCA6, lymphoid-spec Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using HELLS antibody (orb1274441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and HELLS knockout (KO) HeLa cells, using HELLS antibody (orb1274441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded mouse testis using HELLS Antibody (orb1274441) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug HELLS antibody (orb1274441). Western blot was performed from the immunoprecipitate using HELLS antibody (orb1274441) at a dilution of 1:1000.
