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HELLS Antibody
SKU: orb1274441
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human HELLS (NP_060533.2). |
| Target | HELLS |
| Molecular Weight | Observed: 120kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−ICF4, LSH, Nbla10143, PASG, SMARCA6, lymphoid-specific helicase, SWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 6, proliferation-associated SNF2-like protein
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Western blot analysis of extracts of various cell lines, using HELLS antibody (orb1274441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and HELLS knockout (KO) HeLa cells, using HELLS antibody (orb1274441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded mouse testis using HELLS Antibody (orb1274441) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug HELLS antibody (orb1274441). Western blot was performed from the immunoprecipitate using HELLS antibody (orb1274441) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
HELLS
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IP
Immunoprecipitation
HELLS Antibody (orb1274441)
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