Helicobacter Pylori antibody

SKU: orb2276249

Description

Helicobacter Pylori antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Gastric tissue with known helicobacter pylori infection should show a distinct staining of bacteria on the surface and/or within glands. Negative Control: Gastric tissue: Normal epithelial and stromal cells should not stain. Cellular Localization: Cell surface. Cytoplasm. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: MSVA-466M
• Immunogen: Recombinant Helicobacter pylori Catalase protein fragment (around aa323-445) (Exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H. pylori