HDAC9 Antibody (C-term)

• Catalog Number: orb1938454
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB2742
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 111297 Da
• Target: This HDAC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 503-533 amino acids from the C-terminal region of human HDAC9.
• UniProt ID: Q9UKV0
• NCBI: NP_001191074.1, NP_001191077.1, NP_001191073.1, NP_848510.1, NP_848512.1, NP_001191075.1, NP_001191076.1, NP_478056.1, NP_055522.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Histone deacetylase 9, HD9, Histone deacetylase 7B
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Both anti-HDAC9 N-term and C-term Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone. Bottom figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.