HDAC8 Antibody, KO Validated

• Catalog Number: orb1274442
• Category: Antibodies
• Description: HDAC8 Antibody, KO Validated
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 42kDa
• Target: HDAC8
• UniProt ID: Q9BY41
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: HDAC8, HDACL1, RPD3, HD8, WTS, CDA07, CDLS5, MRXS6
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HDAC8 Antibody (orb1274442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from normal (control) and HDAC8 knockout (KO) HeLa cells, using HDAC8 antibody (orb1274442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat testis using HDAC8 antibody (orb1274442) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat spinal cord using HDAC8 antibody (orb1274442) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using HDAC8 antibody (orb1274442) at dilution of 1:100 (40x lens).