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HDAC8 Antibody, KO Validated

Catalog Number: orb1274442

DispatchUsually dispatched within 5-10 working days
$ 960.00
Catalog Numberorb1274442
CategoryAntibodies
DescriptionHDAC8 Antibody, KO Validated
TargetHDAC8
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid
Concentrationbatch dependent
Buffer/PreservativesLiquid
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1).
UniProt IDQ9BY41
MWObserved: 42kDa
Tested applicationsIF, IHC, WB
Application notesWB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesHDAC8, HDACL1, RPD3, HD8, WTS, CDA07, CDLS5, MRXS6
NoteFor research use only
HDAC8 Antibody, KO Validated

Western blot analysis of extracts of various cell lines, using HDAC8 Antibody (orb1274442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

HDAC8 Antibody, KO Validated

Western blot analysis of extracts from normal (control) and HDAC8 knockout (KO) HeLa cells, using HDAC8 antibody (orb1274442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

HDAC8 Antibody, KO Validated

Immunohistochemistry of paraffin-embedded rat testis using HDAC8 antibody (orb1274442) at dilution of 1:100 (40x lens).

HDAC8 Antibody, KO Validated

Immunohistochemistry of paraffin-embedded rat spinal cord using HDAC8 antibody (orb1274442) at dilution of 1:100 (40x lens).

HDAC8 Antibody, KO Validated

Immunohistochemistry of paraffin-embedded human breast cancer using HDAC8 antibody (orb1274442) at dilution of 1:100 (40x lens).