HDAC6 Antibody (C-term)

• Catalog Number: orb1938482
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB5653
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, IHC-P: 1:100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 131419 Da
• Target: This HDAC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1182-1215 amino acids from the C-terminal region of human HDAC6.
• UniProt ID: Q9UBN7
• NCBI: NP_006035.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Histone deacetylase 6, HD6, HDAC6, KIAA0901
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-HDAC6 Antibody (C-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: T47D whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 131 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis on paraffin-embedded Human testis tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.