HDAC2 Rabbit pAb Discontinued

SKU: orb135636

Description

Rabbit polyclonal antibody to HDAC2

Research Area

Cardiovascular Disease, Epigenetics, Stem Cells

Images & Validation

• Tested Applications: ELISA, IHC-P, IP, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:20-50
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 390-488 of human HDAC2 (NP_001518.3).
• Molecular Weight: 60kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti RPD3 antibody, anti YAF1 antibody

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using HDAC2 Rabbit pAb (orb135636) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse heart using HDAC2 Rabbit pAb (orb135636) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat kidney using HDAC2 Rabbit pAb (orb135636) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 200 µg extracts of K562 cells using 1 µg HDAC2 antibody (orb135636). Western blot was performed from the immunoprecipitate using HDAC2 antibody (orb135636) at a dilution of 1:1000.

Western blot analysis of various lysates using HDAC2 Rabbit pAb (orb135636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Quick Database Links

UniProt

Q92769

Fatemeh Rajabinasab1 Pooya Hajimirzaei1 Fatemeh Ramezani1 Fariborz Moayer2 Fazel Gorjipour1 Alireza Nikoofar1 Leila Hasanzadeh1 Michael R Hamblin3 Atousa janzadeh1 Reza Paydar Lung protective effect of Apigenin against X-ray radiation: an experimental study researchsquare.com, (2024)

PubMed:

Applications

WB

Reactivity

Rat

Ahmad Mostaar 1, Zahra Behroozi 2, Ali MotamedNezhad 3, Sourosh Taherkhani 4, Negin Mojarad 5, Fatemeh Ramezani 6, Atousa Janzadeh 7, Pooya Hajimirzaie The effect of intra spinal administration of cerium oxide nanoparticles on central pain mechanism: An experimental study J Bioenerg Biomembr, (2024)

PubMed: 39102102

Applications

WB

Reactivity

Rat

Majid Kazemi 1, Ali Motamed Nezhad 2, Razieh Hajisoltan 3, Fatemeh Ramezani 3, Soroush Taherkhani 4, Fariborz Moayer 5, Faraz Moayer 6, Atousa Janzadeh 2, Ali Dadseresht 1, Reza Ahadi 1, Negin Mojarad 7, Mina Eftekharzadeh 1, Fazel Gorjipour 3 Photobiomodulation therapy: Location-dependent effects on memory and recovery in male adult rats with spinal cord injury J Neuropathol Exp Neurol, (2025)

PubMed: 40447286

Ali Motamed Nezhad, Zahra Behroozi, Keihan Kookli, Alireza Ghadaksaz, Seyedalireza Moghaddas Fazeli, Ali Moshiri, Fatemeh Ramezani, Molood Gooniband Shooshtari & Atousa Janzadeh Evaluation of photobiomodulation therapy (117 and 90s) on pain, regeneration, and epigenetic factors (HDAC 2, DNMT3a) expression following spinal cord injury in a rat model PPS, 103 (2023)

PubMed: