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HDAC2 Antibody
SKU: orb1258301
Featured
Description
Research Area
Cell Biology, Immunology & Inflammation, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Monkey, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 390 to the C-terminus of human HDAC2 (NP_001518.3). |
| Target | HDAC2 |
| Molecular Weight | Observed: 60kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HDAC2 Antibody: HD2, RPD3, YAF1, Histone deacetylase 2, HD2
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Western blot analysis of extracts of various cell lines, using HDAC2 antibody (orb1258301) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody (orb1258301) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody (orb1258301) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody (orb1258301) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using HDAC2 antibody (orb1258301). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of K562 cells using 1 ug HDAC2 antibody (orb1258301). Western blot was performed from the immunoprecipitate using HDAC2 antibody (orb1258301) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
HDAC2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
HDAC2 Antibody (orb1258301)
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