HDAC2 Antibody

• Catalog Number: orb1258301
• Category: Antibodies
• Description: HDAC2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 390 to the C-terminus of human HDAC2 (NP_001518.3).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 60kDa
• Target: HDAC2
• UniProt ID: Q92769
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HDAC2 Antibody: HD2, RPD3, YAF1, Histone deacetyla
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HDAC2 antibody (orb1258301) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody (orb1258301) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody (orb1258301) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody (orb1258301) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using HDAC2 antibody (orb1258301). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of K562 cells using 1 ug HDAC2 antibody (orb1258301). Western blot was performed from the immunoprecipitate using HDAC2 antibody (orb1258301) at a dilution of 1:1000.