HDAC1 Antibody (N-term)

• Catalog Number: orb1788401
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Other
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: 70-99 aa
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 55103 Da
• Target: This HDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the N-terminal region of human HDAC1.
• UniProt ID: Q13547
• NCBI: NP_004955.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: HDAC1; RPD3L1; Histone deacetylase 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-HDAC1 Antibody (N-term) at 1:1000 dilution. Lane 1: mouse uterus lysates. Lane 2: MCF-7 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 60 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of MCF-7 cell stained with HDAC1 Antibody (N-term).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HDAC1 immunoreactivity is localized to Nucleus significantly.