HDAC1 Antibody (C-term)

SKU: orb1938525

Description

HDAC1 Antibody (C-term)

Research Area

Cardiovascular Research, Cell Biology, Infectious Disease & Virology, Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB - 1:1000, IF - 1:10-50
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 55103 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

RPD3L1

All lanes: Anti-HDAC1 Antibody (K464) at 1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: Jurkat whole cell lysates. Lane 3: K562 whole cell lysates. Lane 4: mouse thymus lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of Hela cell stained with HDAC1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). HDAC1 immunoreactivity is localized to Nucleus significantly.

The anti-HDAC1 Pab is used in Western blot to detect HDAC1 in ZR-75-1 cell lysate.

The anti-HDAC1 Pab was used in Western Blot to detect HDAC1 in HEK293 cells.Knockdown of HDAC1 using siRNA against HDAC1 showed a significant decrease of HDAC1 protein using this anti-HDAC1 Pab in HEK293 cells.

Quick Database Links

UniProt

Q13547