HDAC1 Antibody (C-term)

• Catalog Number: orb1938525
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB5642
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 55103 Da
• Target: This HDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 449-482 amino acids from the C-terminal region of human HDAC1.
• UniProt ID: Q13547
• NCBI: NP_004955.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Histone deacetylase 1, HD1, HDAC1, RPD3L1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-HDAC1 Antibody (K464) at 1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: Jurkat whole cell lysates. Lane 3: K562 whole cell lysates. Lane 4: mouse thymus lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of Hela cell stained with HDAC1 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). HDAC1 immunoreactivity is localized to Nucleus significantly.

The anti-HDAC1 Pab is used in Western blot to detect HDAC1 in ZR-75-1 cell lysate.

The anti-HDAC1 Pab was used in Western Blot to detect HDAC1 in HEK293 cells.Knockdown of HDAC1 using siRNA against HDAC1 showed a significant decrease of HDAC1 protein using this anti-HDAC1 Pab in HEK293 cells.