You have no items in your shopping cart.
Cart summary
| Catalog Number | orb768596 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Hck (phospho-Tyr410) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HCK around the phosphorylation site of Tyr410. AA range:381-430 |
| MW | 60kD |
| Tested applications | ELISA, IF, IHC-P |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-HCK antibody, anti-Tyrosine-protein kinase HC Read more... |
| Note | For research use only |
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HCK (Phospho-Tyr410) Antibody.
Immunofluorescence analysis of HeLa cells, using HCK (Phospho-Tyr410) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
