HCG-alpha Antibody

SKU: orb1823168

Description

Human chorionic gonadotropin antibody (hCG) is a glycoprotein hormone synthesized in syncytiotrophoblastic cells of placenta and in certain trophoblastic tumors. The hormone-specific alpha chains have molecular weights of 13 kDa. HCG is found in moles and choriocarcinoma, chorionic components of germ cell tumors, and syncytiotrophoblast like cells in seminoma/dysgerminoma and embryonal carcinoma. In diagnostic pathology, hCG is a useful marker for classification of germ cell tumors, identification of extragonadal germ cell tumors.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the HCG-alpha antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: hCGa/7874
• Immunogen: A recombinant partial protein sequence (within amino acids 1-116) from the human protein was used as the immunogen for the HCG-alpha antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human placental tissue with HCG-alpha antibody (clone hCGa/7874). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free HCG-alpha antibody (clone hCGa/7874) as confirmation of integrity and purity.

Quick Database Links

UniProt

P01215