HBP1 Rabbit Polyclonal Antibody

• Catalog Number: orb157440
• Category: Antibodies
• Description: HBP1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HBP1 (421-514/514aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58 kDa
• Target: HBP1
• UniProt ID: O60381
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: FLJ16340; HBP 1; HBP1_HUMAN; High mobility group b
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HBP1) Polyclonal Antibody, Unconjugated (orb157440) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Protein: intestine (mouse) lysates at 40 ug, Primary: Anti-HBP1 (orb157440) at 1:200, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000, ECL excitated the fluorescence, Predicted band size: 58kD, Observed band size: 58kD.

Sample: Lane 1: Human Hela cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human THP-1 cell lysates, Primary: Anti-HBP1 (orb157440) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 60 kD.

Sample: placenta (mouse) Lysate at 40 ug, Uterus (Mouse) Lysate at 40 ug, bone (Mouse) Lysate at 40 ug, Primary: Anti-HBP1 (orb157440) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 63 kD.