HAVCR1 Antibody

• Catalog Number: orb1240149
• Category: Antibodies
• Description: HAVCR1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-TIM-1 antibody (orb1240149) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-1. The immunogen is located within amino acids 50 - 100 of TIM-1.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 - 8 μg/mL (overnight incubation at 4˚ C); IHC-P: 10 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 44kD Observed: 60kD (Post-modification: 4 N-linked glycosylation)
• Target: HAVCR1
• UniProt ID: Q96D42
• NCBI: NP_036338
• Storage: TIM-1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: TIM-1 Antibody: TIM, KIM1, TIM1, HAVCR, KIM-1, TIM
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: TIM-1 orb1240149 (8 µg/mL), overnight incubation at 4°C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: TIM-1 orb1240149 (8 µg/mL), TIM-1 orb1238784 (1 µg/mL), beta-actin (1 µg/mL), and GAPDH (0.02 µg/mL), overnight incubation at 4°C (orb1240149) or 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Validation with TIM-1 siRNA Knockdown in Hela Cells. HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 µg of HeLa whole cell lysates per lane. Antibodies: orb1240149 (8 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in the Mouse Cell Line. Loading: 15 µg of NIH/3T3 cell lysates. Antibodies: TIM-1 orb1240149 (8 µg/mL), overnight incubation at 4°C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of TIM-1 in Human Uterus Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1 with orb1240149 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.

Immunohistochemistry Validation of TIM-1 in Human Uterus Tissue. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TIM-1 antibody (orb1240149) at 10 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.