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Featured
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 4
| Tested Applications | WB |
|---|---|
| Dilution Range | WB - 1:1000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB22492 |
| Immunogen | Synthetic Peptide |
| Target | HAS2 |
| Molecular Weight | 63566 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-Hyaluronan synthase 2 antibody, anti-HAS2 antibody
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HAS2 Antibody (Center) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from mouse heart tissue lysate, using HAS2 Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
Immunohistochemical analysis of paraffin-embedded H. kidney section using HAS2 Antibody (Center). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
HAS2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HAS2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_005319.1 |
|---|
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Higgins, Claire A. et al. Microenvironmental reprogramming by three-dimensional culture enables dermal papilla cells to induce de novo human hair-follicle growth Proc Natl Acad Sci U S A, 110, 19679-19688 (2013)
Applications
IF
Reactivity
Human
Cheng, Xiao-Bo et al. Prognostic impact of hyaluronan and its regulators in pancreatic ductal adenocarcinoma PLoS One, 8, e80765 (2013)
Applications
IHC
Reactivity
Human
HAS2 Antibody (Center) (orb35978)
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