HAND2 Antibody (Center)

SKU: orb1434875

Description

Rabbit polyclonal antibody to HAND2.

Research Area

Cardiovascular Research, Neuroscience

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:10~50, WB: 1:1000
• Reactivity: Human
• Predicted Reactivity: Mouse, Other, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB36759
• Immunogen: Synthetic Peptide
• Target: HAND2
• Molecular Weight: 23666
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Heart- and neural crest derivatives-expressed protein 2, Class A basic helix-loop-helix protein 26, bHLHa26, Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2, dHAND, HAND2, BHLHA26, DHAND

Fluorescent confocal image of Hela cell stained with HAND2 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HAND2 immunoreactivity is localized to Nucleus significantly.

HAND2 Antibody (Center) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the HAND2 antibody detected the HAND2 protein (arrow).

Quick Database Links

Gene Symbol

HAND2

UniProt

P61296

RefSeq (Protein):NP_068808.1