HAND1 Antibody

SKU: orb1535032

Description

HAND1 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IF, IHC, IHC-P, WB
• Dilution Range: Flo (1:100), IF (1:100), IHC, IHC-P (10 µg/ml), WB (1:500)
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 1G10
• Immunogen: Full length human recombinant protein of human HAND1 (NP_004812) produced in E. coli.
• Target: HAND1
• Purification: Protein A/G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% rAlbumin
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HAND1, EHand, Hxt, BHLHa27, Thing1

Anti-HAND1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Anti-HAND1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAND1.

Flow cytometry of HeLa cells, using anti-HAND1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Quick Database Links

Gene Symbol

HAND1