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Catalog Number | orb1535032 |
---|---|
Category | Antibodies |
Description | HAND1 Antibody |
Target | HAND1 |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG2b |
Conjugation | Unconjugated |
Reactivity | Human |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% rAlbumin |
Immunogen | Full length human recombinant protein of human HAND1 (NP_004812) produced in E. coli. |
Tested applications | FC, IF, IHC, IHC-P, WB |
Dilution range | Flo (1:100), IF (1:100), IHC, IHC-P (10 µg/ml), WB (1:500) |
Clone Number | 1G10 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | HAND1, EHand, Hxt, BHLHa27, Thing1 |
Note | For research use only |
Anti-HAND1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-HAND1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAND1.
Flow cytometry of HeLa cells, using anti-HAND1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
IF, WB | |
Human, Porcine, Rabbit, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |