HADH Antibody

• Catalog Number: orb1247408
• Category: Antibodies
• Description: HADH Antibody
• Target: HADH
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-YERGDASKEDID
• UniProt ID: Q16836
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 33kDa band observed in Human Heart, Muscle and Kidney lysates (calculated MW of 34.3kDa according to NP_005318.2). In transfected HEK293 transiently expressing HADH a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HADH, HADHSC, hydroxyacyl-Coenzyme A dehydrogenase
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_005318.2
• Expiration Date: 12 months from date of receipt.

orb1247408 (0.02 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing HADH with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with orb1247408 in the right panel (mock transfection in first and last lanes).