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Catalog Number | orb1255597 |
---|---|
Category | Antibodies |
Description | HACE1 Antibody |
Target | HACE1 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HACE1 (NP_065822.2). |
UniProt ID | Q8IYU2 |
MW | Observed: 102kDa |
Tested applications | IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | HACE1, KIAA1320 |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using HACE1 antibody (orb1255597) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human colon using HACE1 antibody (orb1255597) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HACE1 antibody (orb1255597) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using HACE1 antibody (orb1255597) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using HACE1 antibody (orb1255597) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HACE1 antibody (orb1255597) at dilution of 1:100 (40x lens).
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC | |
Bovine, Canine, Equine, Gallus, Human, Porcine, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |