HA Peptide

SKU: orb1296681

Description

This peptide tag is derived from an influenza hemagglutinin (HA) epitope. It is widely used in molecular biology for detecting, purifying, and tracking recombinant proteins in both in vitro assays and in vivo model systems.

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 92000-76-5
• MW: 1102.15
• Purity: >99.99% (May vary between batches)
• Formula: C₅₃H₆₇N₉O₁₇
• SMILES: CC(C)C(NC(=O)C(CC(O)=O)NC(=O)C(Cc1ccc(O)cc1)NC(=O)C1CCCN1C(=O)C(N)Cc1ccc(O)cc1)C(=O)N1CCCC1C(=O)NC(CC(O)=O)C(=O)NC(Cc1ccc(O)cc1)C(=O)NC(C)C(O)=O
• Target: Influenza Virus,Others
• Solubility: DMSO:10 mM;H2O:65 mg/mL (58.98 mM)

Bioactivity

• In Vitro:
  HA Peptide, derived from human influenza hemagglutinin (HA) amino acids 98–106, is a potent epitope widely utilized for isolating, purifying, detecting, and tracking proteins of interest due to its strong immunoreactivity. It is commonly employed in the separation of tagged proteins from cell culture supernatants and lysates at neutral pH, making it a valuable tool for coimmunoprecipitation and detection via western blotting. Given its small size, HA Peptide minimally impacts the biological activity and functionality of fusion partner proteins. The purification of recombinant HA-tagged proteins is facilitated by a highly specific anti-HA monoclonal antibody immobilized on resin, allowing for separation. These proteins can be eluted using a gentle method with an HA epitope at 1 mg/mL in TBS or through one of the three chemical elutions: 0.1 M glycine (pH 2-2.8), 3 M NaSCN, or 50 mM NaOH. Additionally, incorporating an N-terminal HA Peptide into mammalian expression vectors is crucial for T7 promoter-driven expression in E. coli, independent of trans-acting T7 RNAP. However, research indicates that caspase 3/7-mediated cleavage of HA Peptide abolishes its immunoreactivity, cautioning against its use in studying cell death and apoptotic mechanisms due to potential artifacts.

Storage & Handling

• Storage: keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, HA Peptide, Inhibitor

Compound Identifiers

PubChem CID

14213118