H6PD Antibody

• Catalog Number: orb1257009
• Category: Antibodies
• Description: H6PD Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human H6PD (NP_004276.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 89kDa
• Target: H6PD
• UniProt ID: O95479
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: H6PD, DKFZp686A01246, G6PDH, GDH, MGC87643, CORTRD
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using H6PD Antibody (orb1257009) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using H6PD antibody (orb1257009) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U2OS cells using H6PD antibody (orb1257009). Blue: DAPI for nuclear staining.