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Item 1 of 3
γ-H2AX Antibody
Catalog Number: orb1100614
| Catalog Number | orb1100614 |
|---|---|
| Category | Antibodies |
| Description | anti- γ-H2AX antibody |
| Target | γ-H2AX |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | liquid |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Immunogen | A synthetic phosphorylated peptide around S139 of human H2AX |
| UniProt ID | P16104 |
| MW | 17 kDa |
| Tested applications | IF, IHC, WB |
| Dilution range | WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:100 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | gamma H2A.X,γH2AX, Phospho-H2AX-S139 |
| Note | For research use only |
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with orb1100614 (γ-H2AX Antibody) at dilution of 1:1000. NIH/3T3 cells were treated by UV for 15-30 minutes.
Immunofluorescent analysis of HeLa cells using orb1100614 (γ-H2AX antibody) at dilution of 1:100. HeLa cells were treated by UV for 15-30 minutes at RT.
Immunohistochemistry of paraffin-embedded mouse testis tissue slide using orb1100614 (γ-H2AX Antibody) at dilution of 1:100.
