H2AFX (Ab-139) Antibody

• Catalog Number: orb416662
• Category: Antibodies
• Description: H2AFX (Ab-139) Antibody
• Target: H2AFX
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Purification: Antigen Affinity Purified
• Immunogen: Peptide sequence around site of Ser (139) derived from Human Histone H2AX
• UniProt ID: P16104
• Tested applications: ChIP, ELISA, IF, IHC, WB
• Dilution range: WB:1:500-5000, IHC-P:1:20-200, IF:1:50-200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-Histone H2AX antibody, anti- H2a/x antibody,
• Note: For research use only

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- H2AFX (Ab-139) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PC

Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysateusing H2AFX (Ab-139) antibody

Immunofluorescence staining of Hela using H2AFX (Ab-139) antibody

Immunohistochemistry of paraffin-embedded human breast cancer tissue using H2AFX (Ab-139) antibody

Immunohistochemistry of paraffin-embedded human cervical cancer tissue using H2AFX (Ab-139) antibody