H2AFX Antibody

SKU: orb416662

Description

This H2AFX (Ab-139) Antibody is an unconjugated polyclonal product. It tagets H2AFX using a Peptide sequence around site of Ser (139) derived from Human Histone H2AX as the immunogen. This antibody is suitable for ChIP, ELISA, IF, IHC, WB. Purification: Antigen Affinity Purified.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ChIP, ELISA, IF, IHC, WB
• Dilution range: WB:1:500-5000, IHC-P:1:20-200, IF:1:50-200
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Peptide sequence around site of Ser (139) derived from Human Histone H2AX
• Target: H2AFX
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Disclaimer: For research use only

Alternative Names

Anti-Histone H2AX antibody, anti- H2a/x antibody, anti- Histone H2A.X antibody, anti- H2AFX antibody, anti- H2AX antibody

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- H2AFX (Ab-139) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PC

Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysateusing H2AFX (Ab-139) antibody

Immunofluorescence staining of Hela using H2AFX (Ab-139) antibody

Immunohistochemistry of paraffin-embedded human breast cancer tissue using H2AFX (Ab-139) antibody

Immunohistochemistry of paraffin-embedded human cervical cancer tissue using H2AFX (Ab-139) antibody

Quick Database Links

Gene Symbol

H2AFX

UniProt

P16104