H1N1 Neuraminidase Antibody

• Catalog Number: orb1238382
• Category: Antibodies
• Description: H1N1 Neuraminidase Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Neuraminidase antibody was raised against synthetic peptides from the seasonal influenza (H1N1) Neuraminidase protein and the novel swine influenza Neuraminidase protein.The antibody recognizes both variants.The immunogen is located within the first 50 amino acids of H1N1 Neuraminidase.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NA
• UniProt ID: C4AL28, B1AGS8
• NCBI: ACA33620, ACQ76308
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: H1N1 Neuraminidase Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: H1N1 Neuraminidase Antibody:
• Note: For research use only
• Application notes: WB: 2 μg/mL. Neuraminidase antibody can be used for the detection of the Neuraminidase protein from the seasonal influenza A (H1N1) and the novel swine influenza in ELISA and WB. It will detect 2 ng of free peptide at 1 μg/mL.
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with H1N1 NA Protein. Loading: 50 ng (Lane 1) and 100 ng (Lane 2) H1N1 Neuraminidase recombinant protein. Antibodies: H1N1 NA antibody, orb1238382, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

ELISA Validation with H1N1 NA Protein. Coating Antigen: H1N1 Neuraminidase recombinant protein, 2 µg/mL, incubated at 4°C overnight. Detection Antibodies: H1N1 NA antibody, orb1238382, dilution: 1-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit at 1:10000, incubated at RT for 1 hr.