H-2 Kb/Db mAb (28-8-6S), InVivoPure+

SKU: orb3127242

Description

The anti-H-2 Kb/Db monoclonal antibody (clone 28-8-6S) was purified from supernatant of HB-51TM 28-8-6S from the ATCC.* Clone 28-8-6S is a mouse monoclonal antibody that specifically binds to the murine MHC class I molecules H-2Kb and H-2Db, which are part of the H-2b haplotype commonly found in C57BL/6 mice. These molecules are essential for presenting endogenous peptides to CD8⁺ cytotoxic T lymphocytes, playing a central role in immune surveillance and response to infection and cancer. H-2Kb and H-2Db are critical for presenting intracellular antigens to CD8⁺ T cells. This antibody is widely used in immunological research, including studies on viral immunity, tumor immunology , and neuroimmune interactions. It is also valuable in transgenic mouse models and vaccine development. This antibody has been reported to be used in immunofluorescence, immunohistochemistry, and flow cytometry.

Images & Validation

• Tested Applications: ELISA, FC, WB
• Reactivity: Mouse
• Application Notes:
  Recomended Isotype: Mouse IgG2A k Isotype Control, Recomended Isotype Catalog: orb2568793

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, Kappa
• Clone No.: 28-8-6S
• Immunogen: Spleen cells from C3H.SW mice
• Target: H-2 Kb/Db
• Purity: ≥ 95 % (CGE, reducing conditions) ≤ 5 % aggregates (analytical SEC) Endotoxin level ≤ 1 EU/mg
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
• Concentration: ≥ 1 mg/ mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MHC class I and mouse anti-mouse H-2Kb/H-2Db

SDS-PAGE gel analysis of orb3127242.

SEC-HPLC purity analysis of orb3127242.

Quick Database Links

Gene Symbol

H-2 Kb/Db

UniProt

P01902