GUSB Antibody (Center)

SKU: orb1927969

Description

GUSB Antibody (Center)

Research Area

Cell Biology, Metabolism Research, Microbiology, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: IHC-P - 1:100, WB - 1:2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 74732 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

GUSB Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical analysis on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis on paraffin-embedded Human colon carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-GUSB Antibody (Center) at 1:4000 dilution. Lane 1: HT-29 whole cell lysate. Lane 2: WiDr whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: A2058 whole cell lysate. Lane 5: HL-60 whole cell lysate. Lane 6: Mouse liver tissue lysate. Lane 7: Mouse lung tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 75 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P08236