Guinea Pig IgG (H&L) Antibody Biotin Conjugated

SKU: orb347069

Description

Guinea Pig IgG (H&L) antibody (Biotin)

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Guinea pig
• Application Notes:
  Anti-Guinea Pig (H&L) biotin conjugated antibody generated in goat detects specifically guinea pig IgG (H&L). This secondary biotin conjugated antibody anti-Guinea Pig has been tested by ELISA and is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Antibody anti guinea pig biotin conjugated has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product. 10-20 moles Biotin per mole. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL

  Read more...

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Guinea Pig IgG (H&L) was produced by repeated immunization with guinea pig whole molecule in goat.
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Guinea pig IgG (H&L) Antibody, Gt-a-Guinea pig Biotin conjugated, Guinea pig IgG (H&L) Antibody in goat, Goat IgG (H&L) Biotin conjugated Secondary Antibody.