Guinea Pig IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

• Catalog Number: orb347053
• Category: Antibodies
• Description: Guinea Pig IgG (H&L) antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: HRP
• Reactivity: Guinea pig
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: Anti-Guinea Pig IgG (H&L) peroxidase conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.
• Immunogen: Anti-Guinea Pig IgG (H&L) was produced by repeated immunization with Guinea Pig IgG whole molecule in goat.
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:100,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application notes: Anti-Guinea Pig IgG HRP has been tested by ELISA and western blot and is suitable for immunoblotting, western blot, dot blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Guinea Pig IgG (H&L) Antibody, Gt-a-Guinea Pig IgG
• Note: For research use only

Western Blot of Goat anti-Guinea Pig IgG HRP Antibody. Lane 1: Molecular Weigh Ladder. Lane 2: Guinea Pig IgG. Lane 3: Bovine Serum. Lane 4: Chicken Serum. Lane 5: Goat Serum. Lane 6: Hamster Serum. Lane 7: Horse Serum. Lane 8: Human Serum. Lane 9: Mouse Serum. Lane 10: Rabbit Serum. Lane 11: Rat Serum. Lane 12: Sheep Serum. Lane 13: MW. Loading: 50 ng IgG, Serum at 1:8 dilution. Blocking Buffer: BlockOut orb348644 for 30 min at RT. Secondary Antibody: Gt-a-GP IgG HRP at 1:40000.