Guinea Pig IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

• Catalog Number: orb347052
• Category: Antibodies
• Description: Guinea Pig IgG (H&L) antibody (FITC)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Guinea pig
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.
• Immunogen: Guinea Pig IgG whole molecule
• Tested applications: DOT, FC, FLISA, IF
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Application notes: Anti-Guinea Pig IgG Fluorescein Antibody has been tested by dot blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: goat Anti-Guinea Pig IgG Antibody fluorescein Conj
• Note: For research use only

Dot Blot results of Goat Anti-Guinea Pig IgG Antibody Fluorescein Conjugate. Dots are Guinea Pig IgG: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: none. Secondary Antibody: Goat Anti-Guinea Pig IgG Antibody FITC at 1 ug/ml in orb348637 1hr RT.