GUCY2D Antibody (Center)

• Catalog Number: orb1938101
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19346
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:2000, IHC-P: 1:50~100, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 120059 Da
• Target: This GUCY2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-570 amino acids from the Central region of human GUCY2D.
• UniProt ID: Q02846
• NCBI: NP_000171.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Retinal guanylyl cyclase 1, RETGC-1, Guanylate cyc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-GUCY2D Antibody (Center) at 1:2000 dilution + rat eyeball lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 120 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

GUCY2D Antibody (Center) western blot analysis in Ramos cell line lysates (35 ug/lane). This demonstrates the GUCY2D antibody detected the GUCY2D protein (arrow).

GUCY2D Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of GUCY2D Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Overlay histogram showing Hela cells (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.