GTF3C4 Antibody

SKU: orb1255635

Description

GTF3C4 Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 583-822 of human GTF3C4 (NP_036336.2).
• Target: GTF3C4
• Molecular Weight: Observed: 95kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GTF3C4, general transcription factor IIIC, polypeptide 4 (90kD), OTTHUMP00000022434, TFiiiC2-90, TFIIICdelta, TFIIIC90, TFIII90, MGC138450, KAT12, FLJ21002, polypeptide 4, 90 kDa, GTF3C4, TFIIIC290, TFIIICDELTA

Western blot analysis of extracts of various cell lines, using GTF3C4 antibody (orb1255635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of C6 cells using GTF3C4 Polyclonal Antibody (orb1255635) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using GTF3C4 Polyclonal Antibody (orb1255635) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

GTF3C4

UniProt

Q9UKN8