GST Antibody: Biotin

• Catalog Number: orb413650
• Category: Antibodies
• Description: Mouse monoclonal antibody against GST conjugated to Biotin
• Target: GST
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Biotin
• Reactivity: All
• Concentration: 1 mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Purification: Protein G Purified
• Immunogen: Glutathione-S-Transferase (GST) tagged protein
• UniProt ID: P08515
• MW: 26 kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000); ELISA (1:800)
• Application notes: A 1:1000 dilution of SMC-549 was sufficient for detection of GST in 1 µg of Purified GST tagged PmtA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: 300
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Glutathione S Transferase antibody, Glutathione S-
• Note: For research use only

Western Blot analysis of Schistosoma japonicum Purified GST showing detection of 26 kDa GST protein using Mouse Anti-GST Monoclonal Antibody, Clone 3E2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein. Lane 3: GST. Load: 1 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-GST Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 26 kDa.