GSN Antibody (C-term)

• Catalog Number: orb1929357
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This GSN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 714-743 amino acids from the C-terminal region of human GSN.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P06396
• MW: 85698 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:50~100, FC: 1:10~50
• Clone Number: RB18722
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Gelsolin, AGEL, Actin-depolymerizing factor, ADF,
• Note: For research use only
• NCBI: NP_001121139.1, NP_937895.1, NP_001121138.1, NP_001244959.1, NP_000168.1, NP_001121137.1, NP_001121134.1, NP_001244958.1, NP_001121135.2, NP_001121136.1

GSN Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

GSN Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-GSN Antibody (C-term) at 1:1000-1:2000 dilution. Lane 1: Hela whole cell lysates. Lane 2: mouse spleen lysates. Lane 3: MCF-7 whole cell lysates. Lane 4: human plasma lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 86 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.