GRP94 Antibody / HSP90B1

• Catalog Number: orb749829
• Category: Antibodies
• Description: Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Gp96 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Gp96 has been briefly studied as a prognostic factor in breast cancer.
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: Rat IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the gp96 antibody.
• UniProt ID: P14625
• Tested applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the GRP94 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: SPM249
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This gp96 antibody is available for research use only.
• Note: For research use only

IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with gp96 antibody (SPM249).

Immunofluorescent staining of permeabilized human HepG2 cells with gp96 antibody (clone SPM249, green) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human HepG2 cells with gp96 antibody (clone SPM249); Red = isotype control, Blue = gp96 antibody.