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Catalog Number | orb385913 |
---|---|
Category | Antibodies |
Description | Heat shock protein 90kDa beta member 1, known also as endoplasmin, gp96, grp94 and ERp99, is an HSP90 paralogue that is found in the endoplasmic reticulum. It plays critical roles in folding proteins in the secretory pathway such as Toll-like receptors and integrins. It has been implicated as an essential immune chaperone to regulate both innate and adaptive immunity. [Wiki] |
Species/Host | Rat |
Clonality | Monoclonal |
Clone Number | SRPR90b |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Rat IgG2a, kappa |
Immunogen | Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody. |
Dilution range | Immunohistology (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This GRP94 antibody is available for research use only. |
UniProt ID | P14625 |
Storage | Store the GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Titering of the GRP94 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human breast carcinoma using GRP94 antibody
FACS, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rat | |
Monoclonal | |
Unconjugated |
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