GRP78 Antibody

• Catalog Number: orb67384
• Category: Antibodies
• Description: Mouse Anti-Human GRP78 Monoclonal IgG1
• Target: GRP78
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Bovine, Frog, Fungi, Hamster, Human, Monkey, Mouse, Plant, Rabbit, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Purification: Protein G Purified
• Immunogen: His-tagged human GRP78
• UniProt ID: P20029
• MW: 78kDa
• Tested applications: ELISA, ICC, IF, WB
• Dilution range: WB (1:2000), ICC/IF (1:100)
• Application notes: 0.5 µg/ml of SMC-196 was sufficient for detection of Grp78 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
• Clone Number: 6H4-2G7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 78 kDa glucose regulated protein Antibody, 78 kDa
• Research Area: Cancer Biology, Cell Biology, Protein Biochemistry
• Note: For research use only
• Entrez: 14828
• NCBI: NP_001156906.1
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 6H4-2G7. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.

Western Blot analysis of Human recombinant cell lysate showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 6H4.2G7. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.