GRP78 Antibody: HRP

SKU: orb181921

Description

Mouse monoclonal antibody against GRP78, conjugated to HRP.

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-210 was sufficient for detection of GRP78 in 20 µg of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 3C5-1A4
• Immunogen: Full-length recombinant rat GRP78
• Target: GRP78
• Molecular Weight: 78kDa
• Purification: Protein G Purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

78 kDa glucose regulated protein antibody, 78 kDa glucose-regulated protein antibody, AL022860 antibody, AU019543 antibody, BIP antibody, D2Wsu141e antibody, D2Wsu17e antibody, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody, Endoplasmic reticulum lumenal antibody, Ca2+ binding protein grp78 antibody, FLJ26106 antibody, Glucose Regulated Protein 78kDa antibody, GRP 78 antibody, GRP-78 antibody, GRP78_HUMAN antibody, Heat shock 70 kDa protein 5 antibody, Heat Shock 70kDa Protein 5 antibody, HSCe70 antibody, HSPA 5 antibody, HSPA5 antibody, Immunoglobulin Heavy Chain Binding Protein antibody, Immunoglobulin heavy chain-binding protein antibody, mBiP antibody, MIF2 antibody, Sez7 antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Western Blot analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.

ELISA analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody.

Quick Database Links

Gene Symbol

GRP78

UniProt

P06761

NCBI Gene ID

25617

RefSeq (Protein):NP_037215.1