Cart summary

You have no items in your shopping cart.

GRP78 Antibody: APC

Catalog Number: orb181918

Select Product Size
SizePriceQuantity
100 μg$ 640.00
100 μg Enquire
DispatchUsually dispatched within 2-3 weeks
Catalog Numberorb181918
CategoryAntibodies
DescriptionMouse monoclonal antibody against GRP78, conjugated to APC.
TargetGRP78
ClonalityMonoclonal
Species/HostMouse
IsotypeIgG1 Kappa
ConjugationAPC
ReactivityHuman, Mouse, Rat
Concentration1 mg/ml
Buffer/Preservatives95.64mM Phosphate, 2.48mM MES and 2mM EDTA
PurificationProtein G Purified
ImmunogenFull-length recombinant rat GRP78
UniProt IDP06761
MW78kDa
Tested applicationsICC, IF, IHC
Dilution rangeWB (1:1000), ICC/IF (1:100)
Application notes1 µg/ml of SMC-210 was sufficient for detection of GRP78 in 20 µg of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Clone Number3C5-1A4
StorageConjugated antibodies should be stored according to the product label
Alternative names78 kDa glucose regulated protein antibody, 78 kDa
Read more...
Research AreaCancer Biology, Cell Biology, Protein Biochemistry
Read more...
NoteFor research use only
Entrez25617
NCBINP_037215.1
Expiration Date12 months from date of receipt.
GRP78 Antibody: APC

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

GRP78 Antibody: APC

Western Blot analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.

GRP78 Antibody: APC

ELISA analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody.

  • GRP78 Antibody: APC [orb151308]

    ICC,  IF,  IHC

    Bovine, Canine, Frog, Fungi, Hamster, Human, Monkey, Mouse, Plant, Rabbit, Rat

    Rabbit

    Polyclonal

    APC

    200 μl
  • GRP78 Antibody: APC [orb148007]

    ICC,  IF,  IHC

    Bovine, Frog, Fungi, Hamster, Human, Monkey, Mouse, Plant, Rabbit, Rat

    Mouse

    Monoclonal

    APC

    100 μg
  • GRP78 Antibody: APC [orb181936]

    ICC,  IF,  IHC

    Human, Mouse, Rat

    Mouse

    Monoclonal

    APC

    100 μg
  • KDEL Antibody: APC [orb151342]

    ICC,  IF,  IHC

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    APC

    200 μg
  • GRP78 (Bip) Antibody: APC [orb151963]

    ICC,  IF,  IHC

    Canine, Drosophila, Hamster, Human, Mouse, Rat

    Rabbit

    Polyclonal

    APC

    100 μg