CXCL1 Antibody

SKU: orb1272521

Description

CXCL1 Antibody

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGRO/MGSA (human GRO/MGSA).
• Target: CXCL1
• Purification: Anti-hGRO/MGSA specific antibody was purified by affinity chromatography employing immobilized hGRO/MGSA matrix.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

FSP, GRO1, GROa, MGSA, NAP-3, SCYB1, MGSA-a, GRO, GROA, Growth-regulated alpha protein, C-X-C motif chemokine 1

To detect Human GRO/MGSA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human GRO/MGSA (orb1272520) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human GRO/MGSA.

To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml–0.150 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml–0.150 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

CXCL1

UniProt

P09341

RefSeq:P09341