GRO/MGSA Antibody

• Catalog Number: orb1272521
• Category: Antibodies
• Description: GRO/MGSA Antibody
• Target: CXCL1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGRO/MGSA (human GRO/MGSA).
• UniProt ID: P09341
• Tested applications: ELISA, NeA, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FSP, GRO1, GROa, MGSA, NAP-3, SCYB1, MGSA-a, GRO,
• Note: For research use only
• NCBI: P09341

To detect Human GRO/MGSA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human GRO/MGSA (orb1272520) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human GRO/MGSA.

To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml–0.150 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml–0.150 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.