GRIA4/GLUR4 Antibody

SKU: orb1538102

Description

GRIA4/GLUR4 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (1 - 2 µg/ml)
• Reactivity: Bovine, Canine, Equine, Gallus, Human, Mammal, Monkey, Mouse, Rabbit, Rat
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx. 90 kD band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2 kD according to NP_001070711.1). Recommended concentration: 0.3-1 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-KKLDQREYPGSETP, from the internal region of the protein sequence according to NP_000820.3NP_001070711.2NP_001070712.1.
• Target: GRIA4 / GLUR4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GRIA4, GluA4, GluR-4, GLUR4, GLUR4C, Glutamate receptor type d, GluR-D, GLURD, Glutamate receptor 4

Antibody (0.3 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

GRIA4 / GLUR4