GRIA4/GLUR4 Antibody

• Catalog Number: orb1538102
• Category: Antibodies
• Description: GRIA4/GLUR4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Bovine, Canine, Equine, Gallus, Human, Mammal, Monkey, Mouse, Rabbit, Rat
• Immunogen: Peptide with sequence C-KKLDQREYPGSETP, from the internal region of the protein sequence according to NP_000820.3NP_001070711.2NP_001070712.1.
• Concentration: 0.5 mg/ml
• Dilution range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (1 - 2 µg/ml)
• Conjugation: Unconjugated
• Target: GRIA4 / GLUR4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: GRIA4, GluA4, GluR-4, GLUR4, GLUR4C, Glutamate rec
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B3606 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3606 was determined to be 5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx. 90 kD band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2 kD according to NP_001070711.1). Recommended concentration: 0.3-1 ug/ml.
• Expiration Date: 12 months from date of receipt.

Antibody (0.3 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.