Grape Seed Extract

SKU: orb1223035

Description

Grape Seed Extract is exudate from seeds of the grape plant Vitis vinifera, composed of oils and secondary plant metabolites (BIOFLAVONOIDS and polyphenols) credited with important medicinal properties.

Key Properties

• CAS Number: 84929-27-1
• MW: 590.58
• Purity: >98% (HPLC)
• Formula: C₃₂H₃₀O₁₁
• SMILES: Cc1cc(O)c([C@H]2[C@H](O)[C@H](Oc3cc(O)cc(O)c23)c2ccc(O)c(O)c2)c2O[C@H](c3ccc(O)c(O)c3)[C@](C)(O)Cc12
• Target: NF-κB
• Solubility: In Vitro: DMSO : 6 mg/mL

Bioactivity

• In Vivo:
  Grape seed extract (200 mg/kg; i. g. ; 5 days/wk; for 8 weeks) inhibits HT29 colon carcinoma tumor xenograft growth in athymic nude mice. Grape seed extract inhibits cell proliferation but increases apoptotic cell death in tumors. Grape seed extract enhances Cip1/p21protein levels and poly(ADP-ribose) poly-merase cleavage in tumors. Animal model: Athymic male nu/nu mice, with human colon carcinoma HT29 tumor xenograft. Dosage: 200 mg/kg. Administration: Oral gavage, 5 days/wk, for 8 weeks. Result: Showed time-dependent inhibition of tumor growth.
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• In Vitro:
  Grape seed extract (25-100 μg/mL; 12-48 hours) inhibits growth and induces death in human colorectal cancer cells. Grape seed extract (25-100 μg/mL; 12-24 hours) modulates the protein levels of cell cycle regulatory molecules in HT29 and LoVo cells. Grape seed extract (25-100 μg/mL; 24 hours) causes apoptotic cell death of human colorectal cancer cells. TCell Proliferation Assay Cell line: HT29 cells, LoVo cells. Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL. Incubation time: 12 hours, 24 hours, 48 hours. Result: Inhibited cell growth in a dose- and a time-dependent manner. Cell Cycle Analysis Cell line: HT29 cells, LoVo cells. Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL. Incubation time: 12 hours, 24 hours, 48 hours. Result: Induced cell cycle arrest (a marked increase in Cip1/p21protein level and a decrease in G 1 phase^associated cyclins and cyclin-dependentkinases). Western blot analysis. Cell line: HT29 cells, LoVo cells. Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL. Incubation time: 12 hours, 24 hours. Result: Modulates the protein levels of CDKIs, CDKs, and cyclins. Apoptosis Analysis Cell line: HT29 cells, LoVo cells. Concentration: 25 μg/mL, 50 μg/mL, 100 μg/mL. Incubation time: 24 hours. Result: Showed a significant dose-dependent increase in apoptotic cell population in HT29 cells at 50 μg/mL and 100 μg/mL; all three Concentrations caused a dose-dependent increase in apoptotic cells.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

NF-κB