GRAP2 Antibody

• Catalog Number: orb1247418
• Category: Antibodies
• Description: GRAP2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-GLFPANYVAPMTR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed lysates of cell line Jurkat (calculated MW of 37.9kDa according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GRAP2
• UniProt ID: O75791
• NCBI: NP_004801.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GRAP2, GRID, P38, GADS, GRPL, GrbX, Mona, GRB2L, G
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed lysates of cell line Jurkat (calculated MW of 37.9kDa according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247418 (0.3 ug/ml) staining of Jurkat lysate (30 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing GRAP2 and probed with orb1247418 (mock transfection in first lane).