Granulocyte Marker Antibody [BM-2]

• Catalog Number: orb1252661
• Category: Antibodies
• Description: Granulocyte Marker Antibody [BM-2]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BM-2
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Primate
• Isotype: IgG1
• Immunogen: Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes were used as the immunogen for this Granulocyte Marker antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Granulocyte Marker antibody to be titered up or down for optimal performance.1. No special pretreatment is required for staining of formalin/paraffin tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Granulocyte Marker antibody to be titered up or down for optimal performance.1. No special pretreatment is required for staining of formalin/paraffin tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of tonsil tissue with Granulocyte Marker antibody (BM-2). Note specific cytoplasmic staining.

SDS-PAGE Analysis of Purified, BSA-Free Granulocyte Marker Antibody (clone BM-2). Confirmation of Integrity and Purity of the Antibody.