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Catalog Number | orb248317 |
---|---|
Category | Antibodies |
Description | GM-CSF (Granulocyte/macrophage - Colony-stimulating factor) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. |
Species/Host | Rat |
Clonality | Monoclonal |
Clone Number | BVD2-21C11 |
Tested applications | FACS, IF, IHC-Fr, IP, NeA, WB |
Reactivity | Human |
Isotype | Rat IgG2a, kappa |
Immunogen | Recombinant human protein was used as the immunogen for this antibody. |
Antibody Type | Primary Antibody |
Dilution range | Neutralization Studies (order BSA/sodium azide-free format),Immunoprecipitation: 0.5-1ug/500ug protein lysate,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This GM-CSF antibody is available for research use only. |
Entrez | 1437 |
Storage | Store the GM-CSF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the GM-CSF antibody to be titered up or down for optimal performance. |
Expiration Date | 12 months from date of receipt. |
FACS, IF, IHC-Fr, IP, NeA, WB | |
Human | |
Rat | |
Monoclonal | |
Unconjugated |