GPX3 Antibody

• Catalog Number: orb1247424
• Category: Antibodies
• Description: GPX3 Antibody
• Target: GPX3
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine
• Predicted Reactivity: Canine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-NQFGKQEPGENSE
• UniProt ID: P22352
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 26kDa band observed in Mouse Kidney and Liver lysates (calculated MW of 27.7kDa according to Mouse NP_001077398.1 [isoform1], 25.4kDa according to Mouse NP_032187.2 [isoform 2] and 25.5kDa according to Human NP_002075.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Colon. Recommended concentration: 5ug/ml. Frozen sections of Porcine Kidney shows vesicular staining of the cytoplasm in PCT epithelial cells. Recommended concentration: 20ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GPX3, glutathione peroxidase 3 (plasma), GPx-P, GS
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_002075.2

orb1247424 (1 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247424 (20 ug/ml) staining of PFA-perfused cryosection of Porcine Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining.

orb1247424 (5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247424 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.