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GPX3 Antibody

SKU: orb1247424

Description

GPX3 Antibody

Research Area

Metabolism Research

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman, Mouse, Porcine
Predicted ReactivityCanine, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 26kDa band observed in Mouse Kidney and Liver lysates (calculated MW of 27.7kDa according to Mouse NP_001077398.1 [isoform1], 25.4kDa according to Mouse NP_032187.2 [isoform 2] and 25.5kDa according to Human NP_002075.2). Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Colon. Recommended concentration: 5µg/mL. Frozen sections of Porcine Kidney shows vesicular staining of the cytoplasm in PCT epithelial cells. Recommended concentration: 20µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-NQFGKQEPGENSE
TargetGPX3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

GPX3, glutathione peroxidase 3 (plasma), GPx-P, GSHPx-3, GSHPx-P , extracellular glutathione peroxidase, plasma glutathione peroxidase 3

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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