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Item 1 of 4
GPR45 Antibody (Center)
Catalog Number: orb1937627
| Catalog Number | orb1937627 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB32265 |
| Tested applications | IHC-P, WB |
| Predicted Reactivity | Mouse |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 41967 Da |
| Target | This GPR45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-149 amino acids from the Central region of human GPR45. |
| UniProt ID | Q9Y5Y3 |
| NCBI | NP_009158.3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Probable G-protein coupled receptor 45, PSP24-1, P Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-GPR45 Antibody (Center) at 1:2000 dilution. Lane 1: SH-SY5Y whole cell lysate. Lane 2: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-GPR45 Antibody (Center) at 1:2000 dilution + human cerebellum lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
GPR45 Antibdy (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of GPR45 Antibdy (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
GPR45 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35 ug/lane). This demonstrates the GPR45 antibody detected the GPR45 protein (arrow).
