GPR45 Antibody (Center)

SKU: orb1937627

Description

GPR45 Antibody (Center)

Research Area

Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 41967 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti-GPR45 Antibody (Center) at 1:2000 dilution. Lane 1: SH-SY5Y whole cell lysate. Lane 2: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-GPR45 Antibody (Center) at 1:2000 dilution + human cerebellum lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

GPR45 Antibdy (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of GPR45 Antibdy (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

GPR45 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35 ug/lane). This demonstrates the GPR45 antibody detected the GPR45 protein (arrow).

Quick Database Links

UniProt

Q9Y5Y3