GPR31 Polyclonal Antibody

SKU: orb1420441

Description

Rabbit polyclonal antibody to GPR31.

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, ICC, IF, IHC-Fr, IHC-P, WB

Key Properties

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Host	Rabbit
Clonality	Polyclonal

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Disclaimer	For research use only

Alternative Names

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12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor, 12-(S)-HETE receptor, 12-HETER, G-protein coupled receptor 31, GPR31/12-HETER, GPR31

Quality Guarantee

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Sample: HepG2 (human) Cell Lysate at 40 ug. Primary: Anti-ZBTB12 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 35 kD. Observed band size: 36 kD.

Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GPR31) Polyclonal Antibody, Unconjugated at 1: 500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.