GPR31 Polyclonal Antibody

• Catalog Number: orb1420441
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GPR31.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• UniProt ID: O00270
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid rec
• Note: For research use only

Sample: HepG2 (human) Cell Lysate at 40 ug. Primary: Anti-ZBTB12 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 35 kD. Observed band size: 36 kD.

Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GPR31) Polyclonal Antibody, Unconjugated at 1: 500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.