GPR17 Antibody

• Catalog Number: orb1248930
• Category: Antibodies
• Description: GPR17 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Mouse
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: C-EGKTNESSLSAKSE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 45kDa band observed in Human Brain (Cerebellum), and in Rat Brain lysates (calculated MW of 41.0kDa according to Human NP_005282.1 and 37.9kDa according to Rat NP_001065245.1). Recommended concentration: 0.1-0.3ug/ml. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GPR17
• UniProt ID: Q13304
• NCBI: NP_005282.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GPR17, G protein-coupled receptor 17, DKFZp686M182
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 45kDa band observed in Human Brain (Cerebellum), and in Rat Brain lysates (calculated MW of 41.0kDa according to Human NP_005282.1 and 37.9kDa according to Rat NP_001065245.1). Recommended concentration: 0.1-0.3ug/ml. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248930 (0.1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248930 (0.3 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

orb1248930 (10 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.