GPR17 Antibody

SKU: orb1248930

Description

GPR17 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 45kDa band observed in Human Brain (Cerebellum), and in Rat Brain lysates (calculated MW of 41.0kDa according to Human NP_005282.1 and 37.9kDa according to Rat NP_001065245.1). Recommended concentration: 0.1-0.3ug/ml. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EGKTNESSLSAKSE
• Target: GPR17
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

GPR17, G protein-coupled receptor 17, DKFZp686M18273

orb1248930 (0.1 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248930 (0.3 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

orb1248930 (10 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

GPR17

UniProt

Q13304

RefSeq (Protein):NP_005282.1